Module Code - Title:
BY4012
-
MICROBES AS METABOLIC FACTORIES
Year Last Offered:
2025/6
Hours Per Week:
Grading Type:
N
Prerequisite Modules:
BY4013
Rationale and Purpose of the Module:
This module is designed to leverage the knowledge of students that have already been introduced to basic concepts of microbiology, principles, and aseptic techniques.
This module will introduce microbe metabolism, in terms of requirements and end products, and will encompass biotechnological applications in hands-on activities.
Syllabus:
The syllabus is comprised of the following topics:
Introduction to metabolism - nutritional requirements of microbes; primary metabolism of carbohydrates, nitrogen and lipids; primary and secondary metabolite production including aspects of pathogens and pathogenicity; mechanism of formation, induction over microbial growth cycle and mode of action of antibiotics, bacteriocins, and biotransformation of intermediates into antibiotics; bioinformatical tools to predict functions and metabolic products
Lactic acid bacteria (LAB) as metabolic factories - Species and strain characteristics, and their application in industrial food production; metabolic end-products and their contribution to flavour formation/quality in fermented foods.
Yeast as metabolic factories - Species and strain characteristics, and their application in industrial production; end products in industrial fermentations.
Moulds as metabolic factories - Species and strain characteristics, and their application in industrial production; end products in industrial fermentations, cheese, industrial enzyme production, waste degradation; Moulds in biofuel and pharmaceutical processes
Microbiome based therapeutics (MBT)
Learning Outcomes:
Cognitive (Knowledge, Understanding, Application, Analysis, Evaluation, Synthesis)
On successful completion of this module, students will be able to:
- Define and differentiate the fundamental characteristics of major groups of lactic acid bacteria, yeasts and moulds.
- Recognize and explain the key requirements for bacterial growth and the formation of the end-product of interest
- Apply the understanding of microbe metabolism to predict outcomes of microbiological interactions in industrial applications
- Discuss the significance of "microbes as metabolic factories" in biotechnology, food chain and health
Affective (Attitudes and Values)
On successful completion of this module, students will be able to:
- Appreciate how the knowledge of microbes' metabolism and their products can be used in favour of a sustainable society.
- Value the biotechnological applications of "Microbes as Metabolic Factories"
Psychomotor (Physical Skills)
On successful completion of this module, students will be able to:
- Perform microbiology experiments, analyse data and results, discuss and report the findings.
- Plan and modify parameters in microbial fermentation processes, and work cooperatively in groups to compare and contrast findings.
How the Module will be Taught and what will be the Learning Experiences of the Students:
The module is delivered in the form of 1hr lecture (in 2 sessions) and a 2-hour laboratory (in one session).
The students will acquire knowledge of relevant concepts, principles, equipment and methodologies. Develop the ability to evaluate and interpret relevant data both qualitative and quantitative and the capacity to use a variety of methods to communicate relevant information cogently and analytically.
The lab report will require a review of scientific literature. News breaking updates on microbiology will be communicated to the students.
Research Findings Incorporated in to the Syllabus (If Relevant):
Prime Texts:
Willey, J.M., Sherwood, L., Woolverton, C.J. (2017)
Prescott's Microbiology, 10th ed
, McGraw-Hill Education: New York, NY
Madigan, M.T., Bender, K.S., Buckley, D.H., Sattley, W.M., Stahl, D.A. (2022)
Brock Biology of Microorganisms, 16th ed
, Pearson: Harlow
Cappuccino, J., Sherman, N. (2013)
Microbiology: Pearson New International Edition: A Laboratory Manual, 10th ed
, Pearson Education Limited
Other Relevant Texts:
Hutkins, R.W. (2019)
Microbiology and Technology of Fermented Foods, 2nd
, IFT Press: Hoboken, NJ,USA.
Neva¿rez-Moorillo¿n, G.V., Prado-Barragan, A., Marti¿nez-Herna¿ndez, J.L., Aguilar, C.N. (2020)
Food Microbiology and Biotechnology¿: Safe and Sustainable Food Production
, Apple Academic Press: Burlington, ON, Canada¿
Hui, Y.H., Evranuz, E.O., Arroyo-Lopez, F.N. (2012)
Handbook of Plant-Based Fermented Food and Beverage Technology, 2nd ed
, CRC Press: Boca Raton, Florida,USA
Mitchell, D.A., Krieger, N., Berovic, M. (2006)
Solid-State Fermentation Bioreactors Fundamentals of Design and Operation, 1st ed
, Springer: Berlin Heidelberg,Germany
Bhukya, B., Tangutur, A.D (2017)
Microbial Biotechnology¿: Technological Challenges and Developmental Trends
, Apple Academic Press: Oakville, ON
Programme(s) in which this Module is Offered:
BSFSHEUFA - FOOD SCIENCE AND HEALTH
BSBIOSUFA - BIOSCIENCE
BSINBIUFA - INDUSTRIAL BIOCHEMISTRY
Semester(s) Module is Offered:
Spring
Module Leader:
fabiana.sarda@ul.ie